Tag

Part:BBa_K4159006

Designed by: Melissa Hendrén   Group: iGEM22_Aalto-Helsinki   (2022-07-14)


Avi tag

Avi-tag

Description: Avi-tag for downstream processing of proteins. The biotin tag will bind specifically to avidin. The avi-tag sequence is taken from the pET_Biotin_His6_GFP_LIC_cloning_vector_(H6-msfGFP) plasmid.


References: pET Biotin His6 GFP LIC cloning vector (H6-msfGFP) was a gift from Scott Gradia (Addgene plasmid # 29725 ; http://n2t.net/addgene:29725 ; RRID:Addgene_29725)


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None