Tag

Part:BBa_K4159004

Designed by: Melissa Hendrén   Group: iGEM22_Aalto-Helsinki   (2022-07-14)


6xHis-tag

6xHis-tag that can be used before the gene of interest. The tag was added between the RBS and the DAPRin sequence.

Characterization: DARPins can be easily purified with the Ni-NTA purification kit when they have this His-tag connected to them. We have done ribosome display as the binding assay for our DARPins, including this His-tag, as well as purified DARPins with the His-tag

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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