Regulatory

Part:BBa_K415513

Designed by: Joy Jiao, Laura Deming, Adrian Slusarczyk   Group: iGEM10_MIT   (2010-10-27)

pWRE_SV40 L4R1 MammoBlock Entry Vector

L4R1 MammoBlock Promoter Vector. Synthetic construct consisting of a concatenation of the following elements:

  • The Wnt-Responsive Element from the Lef1 promoter
  • SV40 minimal constitutive promoter.

Wnt has been shown to be heavily involved in many transduction pathways induced by mechanical stress. The WRE motif is suspected of being able to offer mechanosensitivity to synthetic promoters, particularly to adhesion and strain.

References

Hughes-Fulford, M. "Signal Transduction and Mechanical Stress." Science STKE 2004.249 (2004). Print. Nelson, W. J. "Convergence of Wnt, B-Catenin, and Cadherin Pathways." Science 303.5663 (2004): 1483-487. Print.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 6
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6
    Illegal BglII site found at 155
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 6
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 6
    Illegal NgoMIV site found at 126
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None