Regulatory

Part:BBa_K415508

Designed by: Laura Deming, Joy Jiao, Adrian Slusarczyk   Group: iGEM10_MIT   (2010-10-27)

phEF1a L4R1 MammoBlock Entry Vector

L4R1 Mammoblock Promoter Vector containing human elongation factor 1a (hEF1a) promoter. hEF1a is a low level constitutive promoter.

Characterization

Figure 1. Fluorescence of HEK293FT cells 7 days post infection with hEF1a_EGFP construct.
HEK293 FT cells were infected with replication-deficient HIV particles carrying the hEF1a_EGFP construct. Fluorescent micrographs were taken 7 days after the procedure and show low level constitutive expression from EGFP under the control of the hEF1a promoter.

                                                                                                                                                                                                                                     


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 1235
    Illegal PstI site found at 361
    Illegal PstI site found at 866
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 1235
    Illegal PstI site found at 361
    Illegal PstI site found at 866
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 1235
    Illegal BglII site found at 615
    Illegal XhoI site found at 1014
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 1235
    Illegal PstI site found at 361
    Illegal PstI site found at 866
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 1235
    Illegal PstI site found at 361
    Illegal PstI site found at 866
    Illegal NgoMIV site found at 749
    Illegal AgeI site found at 127
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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