Translational_Unit

Part:BBa_K415204

Designed by: Grant Robinson   Group: iGEM10_MIT   (2010-08-20)


B0034 : cymR Repressor Protein (Strong cymR Translational Unit)

This is a translational unit consisting of a strong RBS and the cymR repressor protein; cymR binds tightly and efficiently to the cmt operator, but changes conformation and detaches from DNA when it forms a complex with cumate (isopropybenozate). This part allows for strong translation of mature RNA containing the cymR coding sequence.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 375
    Illegal XhoI site found at 156
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 58


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Categories
Parameters
None