Regulatory

Part:BBa_K4151019

Designed by: JIUN-JEN, TSAI   Group: iGEM22_CSMU_Taiwan   (2022-09-19)


F2A

F2A is one of the members of the 2A self-cleaving peptide family, a class of 18–22 aa-long peptides, which can induce ribosomal skipping during the translation of a protein in a cell. F2A separates our target protein and RFP during transcription. If the sequence conjugates, it might form a fusion protein which can interrupt the process of protein folding and therefore affect the protein's function. By inserting F2A between RFP and AID, we ensure that fusion protein is less likely to be expressed, which leads to a higher possibility of properly folded AID.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None