Protein_Domain

Part:BBa_K4151017

Designed by: JIUN-JEN, TSAI   Group: iGEM22_CSMU_Taiwan   (2022-09-14)


rtTA

Since AID is a deaminase that would induce point mutation, the expression of AID should be tightly regulated. We incorporate the Tet-on system, an inducible protein expression system, into our design to achieve this purpose. When tetracycline is added, it combines with rtTA(reverse tetracycline-controlled transactivator) to form a rtTA-tetracycline complex, which can recognize and attach to the TetO sequence and express our target protein. By this approach, our construct can regulate the amount of AID expressed.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 4
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 4
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 4
    Illegal NgoMIV site found at 621
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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