Plasmid_Backbone

Part:BBa_K415049

Designed by: Shawn Pan   Group: iGEM10_MIT   (2010-10-26)


Cm Backbone with pBR322 Derived Origin

This part is a biobrick backbone with chloramphenicol resistance and pBR322 derived origin. The copy number is expected to be around 20. The characterization of this part is currently happening, and more data will be uploaded soon.

Note: The backbone contains an extra VR sequencing site from the origin K415039. This site will be removed in the final version pSB6C5. Also, cells containing this plasmid grow slower than usual.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2405
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 2411
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2405
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 2405
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 2405
    Plasmid lacks a suffix.
    Illegal XbaI site found at 2420
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal SapI site found at 1414


[edit]
Categories
Parameters
None