Composite

Part:BBa_K415011

Designed by: Grant Robinson   Group: iGEM10_MIT   (2010-07-06)


PtetR : RBS : LuxR : Term : PluxR/cI-OR : RBS : mCherry : Term : Plux/cI-OR : RBS : LuxI

This composite contains parts BBa_K415010 and BBa_C0261. K415010 is a circuit which produces mCherry regulated by the LuxR/cI-OR promoter and LuxR regulated by the TetR promoter; in addition, the circuit concludes with a second LuxR/cI-OR promoter for further control and BioBrick addition. BBa_C0261 is a LuxI generator, which itself is a C6O3HSL (AHL) generator.

Combined together, this part will produce a signal (mCherry) which is increasingly amplified as time passes and which is induced by the addition of C6O3HSL.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2748
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None