Composite

Part:BBa_K415010

Designed by: Jason Stevens, Andrew Yang   Group: iGEM10_MIT   (2010-06-26)

This is part K415006 with an appended Plux/cI promoter (R0065)

This composite is composed of the parts BBa_K415000 and BBa_S03119. It can produce both luxR and mCherry. The production of luxR is normally constitutive, but can be repressed by TetR and derepressed by aTc. The production of mCherry is activated by c6-AHL and luxR, and repressed by cI. This is a test part that generates a reporter. It also contains an additional R0065 hybrid promoter appended after the LuxR : Term : Term. Please refer to Part:BBa_K415006, which does not contain the additional R0065 hybrid promoter after the LuxR : Term : Term, for relevant information about this part's fluorescence in response to AHL induction and when co-transformed with Collins' toggle pTSMa BioBricked as Part:BBa_K415300.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None