Composite

Part:BBa_K415009

Designed by: Crystal McKenzie   Group: iGEM10_MIT   (2010-06-18)

pLux/cI-OR : RBS-mCherry : Term : P(tetR) : RBS-luxR : Term : pLux

This composite is composed of the parts BBa_K415000 and BBa_F2620. It can produce both luxR and mCherry. The production of luxR is normally constitutive, but can be repressed by TetR and derepressed by aTc. The production of mCherry is activated by c6-AHL and luxR, and repressed by cI.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1986


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Categories
Parameters
None