Composite

Part:BBa_K415007

Designed by: Crystal McKenzie   Group: iGEM10_MIT   (2010-06-18)

pLux/cI-OR : RBS-mCherry : Term : pLux/cI-OR : RBS-luxI : Term : p(tetR) : RBS-luxR : Term

This composite is composed of the parts BBa_K415000 and BBa_I15030. It can produce both luxR and mCherry. The production of luxR is normally constitutive, but can be repressed by TetR and derepressed by aTc. The production of mCherry is activated by c6-AHL and luxR, and repressed by cI. The AHL input signal is amplified by the production of more AHL by luxI.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1742
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None