Composite

Part:BBa_K415003

Designed by: Jason Stevens   Group: iGEM10_MIT   (2010-06-09)

P_const(low/med) : RBS : LuxR : Term : pLux/CI-OR

This composite part is BBa_K145270 (P_const(low/med) : LuxR : Term) forward-inserted in a backbone with BBa_R0065 (pLux/CI-OR), a hybrid promoter. This allows for constitutive expression of LuxR product. Addition of AHL will activate LuxR and the hybrid promoter. CI will inhibit the hybrid promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None