Coding
PcOSM

Part:BBa_K4147000

Designed by: Denisse Chacon Ramirez   Group: iGEM22_Tec-Chihuahua   (2022-09-30)

PcOSM: An osmotin from Piper colubrinum (An antimicrobial peptide)

This osmotin (PcOSM) is a plant defense protein with a molecular weight of ~25 kDa. PcOSM belongs to pathogenesis-related-5 (PR-5) family, which is accumulated in response to both biotic and abiotic stresses [1]. This osmotin has exhibited significant inhibitory activity against the oomycete pathogen Phytophthora capsici. This peptide is able to inhibit fungal growth through inhibition of hyphal growth, spore germination, spore lysis, and reduction in viability of spore [2].


Usage and Biology

This part codes for an osmotin native from Phytophthora resistant wild Piper colubrinum [3]. The codons of this sequence were optimized for expression in E. coli. This gene encodes for a 230 amino acids peptide fused with a 6XHis Tag. PcOSM have shown significant inhibitory activity against P. capsici, which is one of the most devastating pathogens infecting chilli crops [1]. Also, it has shown inhibitory activity against other fungal pathogens as Fusarium oxysporum [3]. Several experiments suggest that the mechanism of action of this osmotin is membrane permeabilization due to the positive charge of the osmotin [3]. Also, it has been hypothesized that the fungal inhibition shown by osmotin is due to the induction of ROS production [1].

PcOSM.png
Figure 1. Action mechanism of PcOSM.

This peptide has also attracted attention as it has shown similarity in functionality to adiponectin, making it also a molecule with therapeutic potential [1, 3, 4]. In addition, it has shown neuroprotective activity and has shown anti-atherosclerotic effects in rat studies [5, 6].


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 579
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 271
  • 1000
    COMPATIBLE WITH RFC[1000]


References

1. Geetha RG, Krishnankutty Nair Chandrika S, Saraswathy GG, Nair Sivakumari A, Sakuntala M. ROS Dependent Antifungal and Anticancer Modulations of Piper colubrinum Osmotin. Molecules. 2021; 26(8):2239. https://doi.org/10.3390/molecules26082239

2. Chowdhury, S., Basu, A. & Kundu, S. Cloning, Characterization, and Bacterial Over-Expression of an Osmotin-like Protein Gene from Solanum nigrum L. with Antifungal Activity Against Three Necrotrophic Fungi. Mol Biotechnol 57, 371–381 (2015). https://doi.org/10.1007/s12033-014-9831-4

3. Mani T, Sivakumar KC, Manjula S. Expression and functional analysis of two osmotin (PR5) isoforms with differential antifungal activity from Piper colubrinum: prediction of structure--function relationship by bioinformatics approach. Molecular biotechnology. 2012;52(3):251–261.

4. Narasimhan ML, Coca MA, Jin J, κ.ά. Osmotin is a homolog of mammalian adiponectin and controls apoptosis in yeast through a homolog of mammalian adiponectin receptor. Molecular cell. 2005;17(2):171–180.

5. Ali, T.; Yoon, G.H.; Shah, S.A.; Lee, H.Y.; Kim, M.O. Osmotin attenuates amyloid beta-induced memory impairment, tau phosphor-ylation and neurodegeneration in the mouse hippocampus. Sci. Rep. 2015, 5, 1–7.

6. Watanabe, T. Therapeutic properties of the new phytochemical osmotin for preventing atherosclerosis. Vessel Plus 2020, 4.

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