Part:BBa_K4146505
pet-lacI-lac-aro10-adh6-T1
This composite element mainly contains two structural sequences. One is aro10, which is used to encode and synthesize Phynpyruvate decarboxylase. The other is adh6, which can help forming 2-phenylethanol, from phenylacetaldehyde.Both sequences can be obtained from Saccharomyces cerevisiae.This element is a key gene element for the engineered bacteria constructed by our team to generate rose flavored 2-phenylethanol from glucose on the scalp surface.
Fig.1 The standard curve for determination of 2-phenylethanol yield.
In the standard curve, as shown in Fig.1,the ordinate is the peak area and the abscissa is the marker concentration.The mobile phase of high performance liquid chromatography is trifluoroacetic acid diluted 1000 times.In our experiment, we took two points, 500 mg/L and 1000 mg/L, as well as zero point. The R square fitted by the standard curve is 1, which shows that the experimental results are fitted very well, and the error can be almost ignored, that is, the measured output is relatively accurate.
Fig.2 The experimental 2-phenylethanol yield.
According to the data read out on the standard curve, we have drawn the curve of 2-PE concentration versus time ,as shown in Fig.2.From the figure, we can see that the concentration of 2-phenylethanol in fermentation is gradually increasing. At 60 hours, the concentration of 2-phenylethanol reached a peak. After that, it began to decline, probably due to the lack of nutrients, and E. coli began to consume 2-phenylethanol to meet its own growth needs.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1526
Illegal EcoRI site found at 2099
Illegal EcoRI site found at 2956
Illegal EcoRI site found at 4994
Illegal SpeI site found at 2441
Illegal SpeI site found at 3945
Illegal PstI site found at 3823 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1526
Illegal EcoRI site found at 2099
Illegal EcoRI site found at 2956
Illegal EcoRI site found at 4994
Illegal SpeI site found at 2441
Illegal SpeI site found at 3945
Illegal PstI site found at 3823 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1526
Illegal EcoRI site found at 2099
Illegal EcoRI site found at 2956
Illegal EcoRI site found at 4994
Illegal BglII site found at 1541
Illegal XhoI site found at 2371 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1526
Illegal EcoRI site found at 2099
Illegal EcoRI site found at 2956
Illegal EcoRI site found at 4994
Illegal SpeI site found at 2441
Illegal SpeI site found at 3945
Illegal PstI site found at 3823 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1526
Illegal EcoRI site found at 2099
Illegal EcoRI site found at 2956
Illegal EcoRI site found at 4994
Illegal SpeI site found at 2441
Illegal SpeI site found at 3945
Illegal PstI site found at 3823
Illegal NgoMIV site found at 1418
Illegal NgoMIV site found at 6107 - 1000COMPATIBLE WITH RFC[1000]
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