Coding

Part:BBa_K4146220

Designed by: Cai xinyi   Group: iGEM22_BUCT   (2022-09-19)


caffeine synthase gene TcAncCS1, a transmethylase

CS stands for caffeine synthase, while the TcAncCS1 ancestral gene is derived from Cocobroma, the duplication of TcAncCS1 at node O leads to TcAncCS2, but the kinetic parameters of TcAncCS2 and its progeny enzymes predict that experts will be favored because the 3 methyl Xanthine does not compete for active site binding. We introduced this plasmid gene to facilitate the synthesis of caffeine in engineered strains, thereby regulating the balance of scalp oil.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 543
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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