Coding
TcCS2

Part:BBa_K4146103

Designed by: Yazhao Liu   Group: iGEM22_BUCT   (2022-09-16)


TcCS2

TcCS2 is used for caffeine synthesis. TcCS2 from C. sinensis has a higher propensity to recognize 3-mX and can convert 3-mX to theobromine. The main caffeine biosynthetic pathway is a sequence consisting of xanthosine→7-methylxanthosine→7-methylxanthine→theobromine→caffeine. Genes encoding N-methyltransferases involved in three of these four reactions have been isolated and the molecular structure of N-methyltransferases investigated.


This gene can be found in E. coli Nissle 1917


This gene is present in part <a href = "https://parts.igem.org/Part:BBa_K4146106"> BBa_K4146106</a>.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 549
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None