Composite

Part:BBa_K4144044

Designed by: Pi Ruiqi   Group: iGEM22_WHU-China   (2022-10-11)


Lac double selector with fluorescence

This double selection contains sacB and KanR, with a mRFP1 indicating the expression of the whole element. SacB encodes the Bacillus subtilis levansucrase, which hydrolyzed the sucrose into levans, which is lethal to gram-negative bacteria. Since our confirmatory experiments KanR provides the recombinant bacteria ability to resist kanamycin. Using sacB as negative selector and KanR as positive one, we are able to devided the ideal recombinant strains of bacteria.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 761
    Illegal AgeI site found at 873
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None