Composite
Part:BBa_K4144044
Designed by: Pi Ruiqi Group: iGEM22_WHU-China (2022-10-11)
Lac double selector with fluorescence
This double selection contains sacB and KanR, with a mRFP1 indicating the expression of the whole element. SacB encodes the Bacillus subtilis levansucrase, which hydrolyzed the sucrose into levans, which is lethal to gram-negative bacteria. Since our confirmatory experiments KanR provides the recombinant bacteria ability to resist kanamycin. Using sacB as negative selector and KanR as positive one, we are able to devided the ideal recombinant strains of bacteria.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 761
Illegal AgeI site found at 873 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |