Part:BBa_K4144005

opMistic is a peptide from B. subtilis that can facilitate fused protein to localize membrane

Mistic (Mstx) is a unique hydrophilic protein from Bacillus subtilis that works as a fusion tag and helps to integrate other exogenous proteins into lipid membranes more easily. When fused to the N-terminus of integral membrane proteins, MstX enables the cargo proteins to fold into their native conformations in the membrane, thus yielding a high-level expression. To improve its translation efficiency, we used the codon optimization tool ExpOptimizer to optimize it into opMistic as a N-terminal signal.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]