RBS
RBS_grac

Part:BBa_K4144001

Designed by: Boshuo Zhou   Group: iGEM22_WHU-China   (2022-10-01)


An intrinsic RBS in pHT01K for inserted fragments to be translated.

This ribosome binding site (RBS) is intrinsic in plasmid vector pHT01K, which locates downstream of Promotor Pgrac inhibited by LacI. With such intrinsic RBS, CDS of interest inserted into MCS can be transcribed and translated with no need to add additional expression elements. With such a RBS, our protein of interest Sam2 can be expressed properly.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
genotypeRBS