Part:BBa_K4141006

H4 sequence from HCR (STAT3-PLK1)

To deliver with safety and efficiency the siRNAs with Theriac we exploited a technique called Hybridization Chain Reaction (HCR). HCR is an isothermal amplification method without the need of enzymes. This technique is based on the recognition and hybridization events between different types of DNA hairpins (in this case 4 different hairpins) in the presence of a specific molecule that initiates the reaction (initiator). We altered these hairpins to carry siRNAs as therapeutic molecules for GBM (Evanko et al. 2004). In detail, Theriac consists of 4 DNA hairpins (HCR-hairpins) complementary to each other; when all hairpins are hybridized in between, two siRNA molecules are released. This part is the 4th of these hairpins (H4) consisting of a sequence complementary to an anti-sense siRNA for PLK1.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]