DNA

Part:BBa_K4141002

Designed by: Anna Anastasiou   Group: iGEM22_Thessaloniki   (2022-10-12)


Initiator sequence from Y-shaped hairpin

Our project this year, Theriac, consists of two different types of hairpins. The first hairpin (Y-shaped) detects at the same time two different cancer biomarkers (miR-21 and miR-10b). In this way discrimination of cancer cells from healthy ones is possible. At the same time by attaching the two microRNAs, Theriac prevents them from acting in favor of cancer cells. Only after the detection of both microRNAS, and through changes in the secondary structure, the initiator is released causing the HCR cycle to begin. This part is the initiator sequence which when released will trigger the HCR procedure and the release of Theriac's therapeutic molecules.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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