Composite

Part:BBa_K414006

Designed by: Matthew Polasko   Group: iGEM10_Nevada   (2010-10-18)


35S Promoter + (Strong Plant RBS + GFP) From K414001

This composite was designed to bring together a constitutive plant promoter (35S) with a fluorescent reporter (GFP) that has a strong plant RBS (Kozak)upstream to maximize translational efficiency. A composite part like this one may be used as an internal control in plant transformations to measure relative, induced expression of an inducible gene.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 191
    Illegal BsaI.rc site found at 1699


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Categories
Parameters
None