Reporter

Part:BBa_K414003

Designed by: Matthew Polasko   Group: iGEM10_Nevada   (2010-09-28)

Strong Plant RBS (Kozak sequence) + mCherry from J06504


In order for mCherry Fluorescent Protein to function properly in plants, a plant-specific ribosome binding site (Kozak sequence)must be added to the 5'region just upstream from the start of translation.The Kozak sequence is AAA AAA AAA ACA and is directly adjacent to the ATG start codon in mCherry.

One point mutation that should be noted is nucleotide 483 of J06504 (mCherry). C -> T is the mutation, but examining the translation of the sequence in the proper reading frame yields no translational discrepancies. Also, no illegal iGEM restriction sites were generated from the point mutation.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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