Part:BBa_K4140028

SEND delivery device

Part Description

Our delivery system SEND platform which formed of 3 domains the first component PEG10 which is GAG protein forms a capsid or vesicle that incircles its own mRNA and self-assembles into virion-like. The second component : VSVg-Fusogen is a typical type III viral fusion protein that, will mediate the delivery of VLPs to target tissues (hepatocytes). Third component: cargo RNA the RNA needed to be carried by the VLPs which is the mRNA of PAH with the addition of 5’ UTR of PEG10 gene to ensure its encapsidation by PEG10 capsid.

Usage

We used a brand new platform to deliver our circuit to the targeted tissue by employing the capacity of PEG10 mRNA to be encapsidated by its expressed GAG protein to deliver the mRNA of PAH to hepatocytes through VLPs.

This was done by adding 5’ and 3’ UTR of PEG10 gene to PAH sequence to guarantee its encapsidation with PEG10 proteins And we employed VSVg-Fusogen to deliver PAH mRNA to the tissue of interest (hepatocytes) as shown in figure 1.

Figure(1) SEND delivery to hepatocytes

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 1045
  Illegal PstI site found at 1747
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1117
  Illegal NheI site found at 3526
  Illegal PstI site found at 1045
  Illegal PstI site found at 1747
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 240
  Illegal BglII site found at 3956
  Illegal BamHI site found at 477
  Illegal BamHI site found at 1160
  Illegal BamHI site found at 4483
  Illegal XhoI site found at 3306
  Illegal XhoI site found at 4193
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 1045
  Illegal PstI site found at 1747
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 1045
  Illegal PstI site found at 1747
  Illegal NgoMIV site found at 3117
  Illegal AgeI site found at 376
  
• 1000
  COMPATIBLE WITH RFC[1000]