Composite
RD29A + Ko

Part:BBa_K414000

Designed by: Elaine Bersaba   Group: iGEM10_Nevada   (2010-09-01)

RD29A Promoter + Strong Plant Kozak (RBS) + RFP

In order for Red Fluorescent Protein to function properly in plants, a plant-specific ribosome binding site (Kozak sequence) must be added to the 5'region just upstream from the start of translation. The Kozak sequence is AAA AAA AAA ACA and is directly adjacent to the ATG start codon in RFP.

Pictured below, you will find the preliminary transformation results of the RD29A-RFP composite in NT BY-2 cells.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 729


[edit]
Categories
//chassis/eukaryote/ntabacum
//function/reporter/fluorescence
Parameters
None