Part:BBa_K4139029

Pro1

This construct begins with Pro1. Pro1 is a protein found in fungal and fungal-like pathogens including Plasmodiophora brassicae. After the Pro1 region we added a His-tag to make purification easier. The use of this part was to have other options other than PbEL04 in Plasmodiophora brassicae. We first made this coding region as a basic part (BBa_K4139020) which we have made as a composite part with a ribosome binding site, promoter, and terminator.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 1728
  Illegal PstI site found at 2028
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 27
  Illegal NheI site found at 50
  Illegal PstI site found at 1728
  Illegal PstI site found at 2028
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 2012
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 1728
  Illegal PstI site found at 2028
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 1728
  Illegal PstI site found at 2028
  Illegal NgoMIV site found at 1922
  Illegal AgeI site found at 529
  
• 1000
  COMPATIBLE WITH RFC[1000]