Part:BBa_K4139028

Rec. CP-MAF

This construct begins with a lysine rich region, with this region being included to enhance conjugation with fluorophore reporter molecules. After this, we have utilized the framework three region of a mouse IGG antibody as a scaffold for our chimeric protein against PbEL04. We have inserted our chimeric protein into the CDR3 region of the framework 3 region of the mouse antibody and have repeated this framework 3 - chimeric protein motif once with the only difference being that the chimeric protein utilized was complementary to PRO1 in the second interaction. After this, we included a 6x His-tag to aid in purification. We first made this coding region as a basic part (BBa_K4139019) which we have made as a composite part with a ribosome binding site, promoter, and terminator.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 980
  Illegal PstI site found at 1238
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 27
  Illegal NheI site found at 50
  Illegal PstI site found at 980
  Illegal PstI site found at 1238
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1312
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 980
  Illegal PstI site found at 1238
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 980
  Illegal PstI site found at 1238
  Illegal AgeI site found at 529
  
• 1000
  COMPATIBLE WITH RFC[1000]