Composite

Part:BBa_K4139028

Designed by: Zachary Robinson & Jason Luddu   Group: iGEM22_Lethbridge   (2023-09-27)


Rec. CP-MAF

This construct begins with a lysine rich region, with this region being included to enhance conjugation with fluorophore reporter molecules. After this, we have utilized the framework three region of a mouse IGG antibody as a scaffold for our chimeric protein against PbEL04. We have inserted our chimeric protein into the CDR3 region of the framework 3 region of the mouse antibody and have repeated this framework 3 - chimeric protein motif once with the only difference being that the chimeric protein utilized was complementary to PRO1 in the second interaction. After this, we included a 6x His-tag to aid in purification. We first made this coding region as a basic part (BBa_K4139019) which we have made as a composite part with a ribosome binding site, promoter, and terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 980
    Illegal PstI site found at 1238
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 27
    Illegal NheI site found at 50
    Illegal PstI site found at 980
    Illegal PstI site found at 1238
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1312
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 980
    Illegal PstI site found at 1238
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 980
    Illegal PstI site found at 1238
    Illegal AgeI site found at 529
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/ecoli
//function/reporter
Parameters
directionForward
proteinCP-MAF
tagHis-Tag