Part:BBa_K4139026

CAPE-GFP

For this construct it starts with enhanced green fluorescent protein (eGFP) followed by our chimeric anti-PbEL04 (CAPE) which is a complementary to PbEL04. We hypothesized that CAPE was complementary to PbEL04 through simulations between the two proteins. Finally we added a His-tag to help with the purification of the protein. The reason for using eGFP is to show if proper binding has occurred. If proper binding has occurred then the protein will glow green when blue light in the 470 nm to 498 nm range. We first made this coding region as a basic part (BBa_K4139013) which we have made as a composite part with a ribosome binding site, promoter, and terminator.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 1767
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 27
  Illegal NheI site found at 50
  Illegal PstI site found at 1767
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 1767
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 1767
  Illegal AgeI site found at 529
  
• 1000
  COMPATIBLE WITH RFC[1000]