Part:BBa_K4139025

CAPE-AFP:

This construct begins with a His-tag, which helps with the purification of the protein. After the His-tag is our chimeric Anti-PbEL04 (CAPE) repeated three times, this was done to increase the change of binding with PbEL04 and overall structure to the chimeric fluorophore probe. Finally, we added a lysine rich region. The lysine rich region is present to bind to colloidal gold particles, which when excited, will fluoresce in visible light giving a signal which will be explored with the Club^2 test strips. We first made this coding region as a basic part (BBa_K4139018) which we have made as a composite part with a ribosome binding site, promoter, and terminator.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 1052
  Illegal PstI site found at 1223
  Illegal PstI site found at 1394
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 27
  Illegal NheI site found at 50
  Illegal PstI site found at 1052
  Illegal PstI site found at 1223
  Illegal PstI site found at 1394
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 1052
  Illegal PstI site found at 1223
  Illegal PstI site found at 1394
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 1052
  Illegal PstI site found at 1223
  Illegal PstI site found at 1394
  Illegal AgeI site found at 529
  
• 1000
  COMPATIBLE WITH RFC[1000]