Part:BBa_K4139022

Full Length PbEL04

This construct was made to have a full version of PbEL04. After identifying two protein targets in Plasmodiophora brassicae, the team needed parts that allowed us to test interactions with the antigens of interest. Here we see full length PbEL04 (our antigen) with the addition of a His-tag to the end to help with purification. Our goal with this protein was to purify the version seen in nature to test the full interaction between it and our COAPE-GFP Protein. PbEL04 is a fibrous protein found in the cell membrane that is secreted from Plasmodiophora Brassicae and aids in the infection of canola. We first made this coding region as a basic part (BBa_K4139012) which we have made as a composite part with a ribosome binding site, promoter, and terminator.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 998
  Illegal PstI site found at 1124
  Illegal PstI site found at 1247
  Illegal PstI site found at 1370
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 27
  Illegal NheI site found at 50
  Illegal PstI site found at 998
  Illegal PstI site found at 1124
  Illegal PstI site found at 1247
  Illegal PstI site found at 1370
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 998
  Illegal PstI site found at 1124
  Illegal PstI site found at 1247
  Illegal PstI site found at 1370
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 998
  Illegal PstI site found at 1124
  Illegal PstI site found at 1247
  Illegal PstI site found at 1370
  Illegal AgeI site found at 529
  
• 1000
  COMPATIBLE WITH RFC[1000]