Part:BBa_K4139011

Truncated PbEL04

This construct starts with a His-tag, this assists with the purification of the protein. After the His-tag we added thioredoxin to further assist with the purification. Thioredoxin helps with the folding of the protein and acts as a solubility tag as well. This is followed by a T.E.V cut site, which allows us to remove the thioredoxin and His-tag once purified. TEV cut site is cleaved off by TEV protease which is a member of the PA clan of chymotrypsin-like proteases. After the TEV cut site is our truncated version of PbEL04. This is just the target epitope of COAP-FP (BBa_K4139015). The use of this construct is to make a version of PbEL04 that is purified easily. We did end up being able to purify this construct.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 498
  Illegal PstI site found at 624
  Illegal PstI site found at 747
  Illegal PstI site found at 870
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 498
  Illegal PstI site found at 624
  Illegal PstI site found at 747
  Illegal PstI site found at 870
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 498
  Illegal PstI site found at 624
  Illegal PstI site found at 747
  Illegal PstI site found at 870
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 498
  Illegal PstI site found at 624
  Illegal PstI site found at 747
  Illegal PstI site found at 870
  
• 1000
  COMPATIBLE WITH RFC[1000]