Primer

Part:BBa_K4138009

Designed by: Carlos Classen   Group: iGEM22_Aachen   (2022-09-28)


2ABH - Primer backward for Gibson Assembly

Usage

The gene 2ABH (BBa_K4138000) was inserted in the pET-28a(+) Vector via Gibson assembly. For the Gibson assembly the target gene and the vector need overlapping gene sequences. These overlapping gene sequences can be achieved trough PCR. Therefore, for the amplification of the insert primers are used which contain an end identical to the vector segment and an end annealing to the insert sequence.

The additional primers used are listed below:

pET-28a(+) backward primer: BBa_K4138011

pET-28a(+) forward primer: BBa_K4138010

2ABH forward primer: BBa_K4138008

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 22
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None