DNA
Spacer

Part:BBa_K4138007

Designed by: Carlos Classen   Group: iGEM22_Aachen   (2022-09-28)


Serien Glycerin Spacer for SOPP3-2ABH

This Spacer was used for the fusion protein of SOPP3-2ABH (BBa_K4138004).

Usage and Biology

Hydrophilic and flexible spacers like Ser/Gly Spacer are popular and common peptide linkers between protein domains. [1] They consist of a repeating of serine an glycine residues. [1] By using a spacer-sequence for fusion proteins, sufficient distance between proteins is ensured, which allows proteins functional folding.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

[1] Understanding and applications of Ser/Gly linkers in protein engineering - PubMed (nih.gov) https://doi.org/10.1016/bs.mie.2020.12.001

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