Part:BBa_K4138006
Serine-Glycine Linker for VVD-CMI
Usage and Biology
Serine-glycine linkers are flexible polypeptide linkers which are used in engineered multidomain proteins. They consist of repeats of glycine and serine residues which prevent the formation of secondary structures and and reduce the interference with the folding and function of the protein domains. [1] In our project, we used the linker in our protein construct VVD-CMI (BBa_K4138005) to fusion the photoreceptor VVD at the N-terminus of the immunity protein CMI.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
[1] van Rosmalen M, Krom M, Merkx M. Tuning the Flexibility of Glycine-Serine Linkers To Allow Rational Design of Multidomain Proteins. Biochemistry. 2017 Dec 19;56(50):6565-6574. doi: 10.1021/acs.biochem.7b00902. Epub 2017 Dec 7. PMID: 29168376; PMCID: PMC6150656.
None |