DNA
Linker

Part:BBa_K4138006

Designed by: Carlos Classen   Group: iGEM22_Aachen   (2022-09-28)


Serine-Glycine Linker for VVD-CMI

Usage and Biology

Serine-glycine linkers are flexible polypeptide linkers which are used in engineered multidomain proteins. They consist of repeats of glycine and serine residues which prevent the formation of secondary structures and and reduce the interference with the folding and function of the protein domains. [1] In our project, we used the linker in our protein construct VVD-CMI (BBa_K4138005) to fusion the photoreceptor VVD at the N-terminus of the immunity protein CMI.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

[1] van Rosmalen M, Krom M, Merkx M. Tuning the Flexibility of Glycine-Serine Linkers To Allow Rational Design of Multidomain Proteins. Biochemistry. 2017 Dec 19;56(50):6565-6574. doi: 10.1021/acs.biochem.7b00902. Epub 2017 Dec 7. PMID: 29168376; PMCID: PMC6150656.

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Categories
Parameters
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