DNA
CMI

Part:BBa_K4138003

Designed by: Carlos Classen   Group: iGEM22_Aachen   (2022-09-28)


Immunity protein CMI-4AEQ

Profile

Name: Colicin-M Immunity Protein (CMI-4AEQ)

Base pairs: 294 bp

Origin: Escherichia coli K-12

Type: Immunity protein

Usage and Biology

The CMI-4AEQ protects the bacteria against its lethal bacteriocins through the co-expression of immunity proteins in the compartment of the colicin destination. [1] In E. coli , the interaction with the CMA immunity protein (CMI) inactivates colicin M (CMA). [1] We used CMI because it is a homodimer that uses phosphate ion as a ligand. [2] CMI is one component of our fusion protein VVD-CMI (BBa_K4138005).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

[1] The crystal structure of the dimeric colicin M immunity protein displays a 3D domain swap - ScienceDirect https://doi.org/10.1016/j.jsb.2012.02.004

[2] RCSB PDB - 4AEQ: Crystal structure of the dimeric immunity protein Cmi solved by direct methods (Arcimboldo) DOI der primary citation: 10.1016/j.jsb.2012.02.004

[edit]
Categories
Parameters
None