Part:BBa_K4138002

Photoreceptor VVD

Profile

Name: Vivid (VVD)

Base pairs: 447 bp

Origin: Neurospora crassa

Blue-light photoreceptor

Usage and Biology

The homodimer VVD, which is produced by the filamentous fungus Neurospora crassa , enables it to adapt to different light conditions. ^[1,3] It normally regulates the production of carotenoids and resets the circadian clock by activating transcription of the clock oscillator protein frequency as well as many other genes. ^[5] It is a photoreceptor that belongs to the LOV (light-oxygen-voltage) protein group and requires FMN (flavin mononucleotid) or FAD (flavin adenin dinucleotid) as cofactors. ^[5] Under the influence of blue light (450 nm) the VVD subunits dimerize within seconds, a covalent bond is formed between a cysteine residue and a flavin group. ^[2,5] The breaking of this bond and the transition to the monomeric form takes several hours. ^[4] In our project, we used the fungal blue-light photoreceptor Vivid (VVD) in one of our fusion constructs VVD-CMI (BBa_K4138005) to enable reversible binding of phosphate to the protein CMI (BBa_K4138003).

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 226
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 226
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 226
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 226
  Illegal AgeI site found at 90
  
• 1000
  COMPATIBLE WITH RFC[1000]

References

[1] Genetically Engineered Photoinducible Homodimerization System with Improved Dimer-Forming Efficiency, dx.doi.org/10.1021/cb400836k

[2] Mechanism Based Tuning of a LOV Domain Photoreceptor, doi:10.1038/nchembio.210

[3] https://www.rcsb.org/structure/3rh8

[4] https://www.optobase.org/switches/LOV-domains/VVD/

[5] Conformational Switching in the Fungal Light Sensor Vivid DOI: 10.1126/science.1137128