Part:BBa_K4138001

Photosensitizer SOPP3

Profile

Name: Singlet Oxygen photosensitizing protein 3 (SOPP3)

Base pairs: 378 bp

Origin: synthetical engineered, derived from Arabidopsis thaliana

Type: Photosensitizer

In our project, we used the singlet oxygen photosensitizing protein 3 (SOPP3) in one of our fusion constructs SOPP3-2ABH (BBa_K4138004) to efficiently disrupt the phosphate binding protein 2ABH (BBa_K4138000).

Usage and Biology

Genetically-encoded photosensitizers induce the production of reactive oxygen species (ROS) such as O₂, O₂^- and H₂O₂ upon light irradiation. The light irradiation with a certain wavelength allows a selective light-induced protein inactivation, degradation and cell death. Photosensitizers do not become cytotoxic before activation by illumination ^[1].

SOPP3 is a variant of the miniSOG protein, which was actively engineered based on LOV2 (Light, Oxygen, and Voltage) domain of phototropin 2, a protein that originated in Arabidopsis thaliana. It was originally designed as a tool for correlative light and electron microscopy, as it both fluoresces and catalyzes the photooxidation of diaminobenzidine used for the detection of ROS. Blue light irradiation induces the synthesis of ROS. The excitation rate is in the range of minutes, allowing a fast reaction to irradiation ^{[2], [3]}.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 187
  Illegal PstI site found at 316
  Illegal PstI site found at 346
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 187
  Illegal PstI site found at 316
  Illegal PstI site found at 346
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 187
  Illegal PstI site found at 316
  Illegal PstI site found at 346
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 187
  Illegal PstI site found at 316
  Illegal PstI site found at 346
  
• 1000
  COMPATIBLE WITH RFC[1000]

References

[1] https://www1.biocat.com/categories/cell-based-analysis/fluorescent-proteins/fluorescent-photosensitizer-vectors (source last accessed: 29.09.2022)

[2] Torra, J., Lafaye, C., Signor, L. et al. Tailing miniSOG: structural bases of the complex photophysics of a flavin-binding singlet oxygen photosensitizing protein. Sci Rep 9, 2428 (2019). https://doi.org/10.1038/s41598-019-38955-3

[3] Christie JM, Salomon M, Nozue K, Wada M, Briggs WR. LOV (light, oxygen, or voltage) domains of the blue-light photoreceptor phototropin (nph1): binding sites for the chromophore flavin mononucleotide. Proc Natl Acad Sci U S A. (1999). https://doi.org/10.1073/pnas.96.15.8779