Coding

Part:BBa_K4134003

Designed by: Jiankai Liu   Group: iGEM22_Nanjing-China   (2022-09-30)


KanR

Forward KanR Protein Coding region only. Needs promoter, RBS, and Terminator in order to provide Kan resistance. It helps us to reflect on whether homologous recombination succeeds or not.

KanR stands for the Kanamycin resistance gene. We kept the coding region and removed its initiation codon so that KanR shares the same initiation codon with BpfA. Then Kanamycin resistance can be used as the basis to judge whether the strain is recombinant that displays silver-binding protein.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None