Part:BBa_K4134002

AgBP2

Description

Ag(I) affinity values of the biomolecules

AgBP2 is a typical silver-binding peptide consisting of 12 amino acids(EQLGVRKELRGV).

The silver-binding protein AgBP2 was selected from a combinatorial peptide display library and it presents a higher Ag(I) affinity value (Ka= 6.30 ±0.5 x10^7) than many other typical silver-binding proteins, such as Ag4 and AgP35.

AgBP2 captures silver ions and paves the way for a new approach to synthesizing silver nanoparticles(AgNPs).And in our project, AgNPs accelerate extracellular electron transfer.

Reference

[1] Li, Xin-Yi, Xiao-Dong Zhou, and Ji-Ming Hu. "A straightforward and reliable evaluation of Ag (I) binding affinity mediated by a peptide ligand for constructing an efficient sensing platform." Talanta 226 (2021): 122089.

[2] Hall Sedlak, Ruth, et al. "Engineered Escherichia coli silver-binding periplasmic protein that promotes silver tolerance." Applied and environmental microbiology 78.7 (2012): 2289-2296.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]