Composite

Part:BBa_K4131013

Designed by: Chun Ying Yu   Group: iGEM22_CCU_Taiwan   (2022-10-10)


The Cold-induced expressing cassette of pfa A and pfa D

In Moritella marina and Shewanella pneumatophori, the pfa gene cluster encodes five open reading frames, namely pfa A, pfa B, pfa C, pfa D, and pfa E. Each gene contains up to several functional domains to perform carboxylation, dehydration, and reduction of the ketoacyl group and hydroxyl groups in the PKS pathway. In Moritella marina, the pfa genes work together to generate DHA, while Shewanella pneumatophori generates EPA. The exact functions of pfa A-D genes are unclear. To the best of our knowledge, the pfa B gene is the key molecule to determine the final product (e.g. EPA and DHA).

This composite part is responsible for the ColdG-inducible pfa A and pfa D protein expression.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 4351
    Illegal SpeI site found at 3866
    Illegal SpeI site found at 5324
    Illegal SpeI site found at 7433
    Illegal PstI site found at 2298
    Illegal PstI site found at 3240
    Illegal PstI site found at 5292
    Illegal PstI site found at 6783
    Illegal PstI site found at 7919
    Illegal PstI site found at 8731
    Illegal PstI site found at 9433
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4351
    Illegal NheI site found at 3823
    Illegal NheI site found at 5618
    Illegal NheI site found at 7307
    Illegal NheI site found at 9656
    Illegal NheI site found at 9979
    Illegal SpeI site found at 3866
    Illegal SpeI site found at 5324
    Illegal SpeI site found at 7433
    Illegal PstI site found at 2298
    Illegal PstI site found at 3240
    Illegal PstI site found at 5292
    Illegal PstI site found at 6783
    Illegal PstI site found at 7919
    Illegal PstI site found at 8731
    Illegal PstI site found at 9433
    Illegal NotI site found at 5681
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4351
    Illegal BglII site found at 4306
    Illegal BglII site found at 4612
    Illegal BglII site found at 5218
    Illegal BglII site found at 7068
    Illegal BglII site found at 7728
    Illegal BglII site found at 8594
    Illegal BamHI site found at 8006
    Illegal XhoI site found at 686
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 4351
    Illegal SpeI site found at 3866
    Illegal SpeI site found at 5324
    Illegal SpeI site found at 7433
    Illegal PstI site found at 2298
    Illegal PstI site found at 3240
    Illegal PstI site found at 5292
    Illegal PstI site found at 6783
    Illegal PstI site found at 7919
    Illegal PstI site found at 8731
    Illegal PstI site found at 9433
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 4351
    Illegal SpeI site found at 3866
    Illegal SpeI site found at 5324
    Illegal SpeI site found at 7433
    Illegal PstI site found at 2298
    Illegal PstI site found at 3240
    Illegal PstI site found at 5292
    Illegal PstI site found at 6783
    Illegal PstI site found at 7919
    Illegal PstI site found at 8731
    Illegal PstI site found at 9433
    Illegal AgeI site found at 4172
    Illegal AgeI site found at 4478
    Illegal AgeI site found at 5084
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2351


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Categories
Parameters
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