Part:BBa_K4131012
The lac-induced expressing cassette of AccBC, AccD1 and AccE
The acetyl-CoA carboxylase of C. glutamicum is composed of a biotin carboxylase (AccBC), a biotin carboxyl carrier protein (AccE), and a transcarboxylase (AccD1). They carboxylated acetyl-CoA into malonyl-CoA, which is necessary for the elongation of nascent fatty acid. The carboxylation occurs in two steps. In the first step, the ATP-dependent biotin carboxylase (AccBC) catalyzes the transfer of a carboxyl group to biotin, which is linked to the biotin carboxyl carrier protein (AccE). In the second step, the transcarboxylase (AccD1) transferred the carboxyl group from carboxybiotin to acetyl-CoA to form malonyl-CoA.
This composite part is responsible for the IPTG-inducible AccBC, AccD1 and AccE protein expression.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 3976
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1568
Illegal XhoI site found at 317 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 925
Illegal NgoMIV site found at 1299
Illegal NgoMIV site found at 1789
Illegal AgeI site found at 663
Illegal AgeI site found at 1165
Illegal AgeI site found at 1948
Illegal AgeI site found at 2330 - 1000COMPATIBLE WITH RFC[1000]
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