Part:BBa_K4131011

The lac-induced expressing cassette of pfa B, pfa C and pfa E (EPA)

In Moritella marina and Shewanella pneumatophori, the pfa gene cluster encodes five open reading frames, namely pfa A, pfa B, pfa C, pfa D, and pfa E. Each gene contains up to several functional domains to perform carboxylation, dehydration, and reduction of the ketoacyl group and hydroxyl groups in the PKS pathway. In Moritella marina, the pfa genes work together to generate DHA, while Shewanella pneumatophori generates EPA. The exact functions of pfa A-D genes are unclear. To the best of our knowledge, the pfa B gene is the key molecule to determine the final product (e.g. EPA and DHA).

This composite part is responsible for the IPTG-inducible pfa B, pfa C, and pfa E protein expression.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 6107
  Illegal XbaI site found at 7363
  Illegal PstI site found at 3288
  Illegal PstI site found at 6044
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 6107
  Illegal NheI site found at 9562
  Illegal PstI site found at 3288
  Illegal PstI site found at 6044
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 6107
  Illegal BglII site found at 7855
  Illegal BamHI site found at 1542
  Illegal XhoI site found at 3983
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 6107
  Illegal XbaI site found at 7363
  Illegal PstI site found at 3288
  Illegal PstI site found at 6044
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 6107
  Illegal XbaI site found at 7363
  Illegal PstI site found at 3288
  Illegal PstI site found at 6044
  Illegal NgoMIV site found at 534
  Illegal AgeI site found at 6863
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 9525
  Illegal SapI site found at 1752