Composite

Part:BBa_K4131010

Designed by: Chun Ying Yu   Group: iGEM22_CCU_Taiwan   (2022-10-10)


The lac-induced expressing cassette of pfa B, pfa C and pfa E (DHA)

In Moritella marina and Shewanella pneumatophori, the pfa gene cluster encodes five open reading frames, namely pfa A, pfa B, pfa C, pfa D, and pfa E. Each gene contains up to several functional domains to perform carboxylation, dehydration, and reduction of the ketoacyl group and hydroxyl groups in the PKS pathway. In Moritella marina, the pfa genes work together to generate DHA, while Shewanella pneumatophori generates EPA. The exact functions of pfa A-D genes are unclear. To the best of our knowledge, the pfa B gene is the key molecule to determine the final product (e.g. EPA and DHA).

This composite part is responsible for the IPTG-inducible pfa B, pfa C, and pfa E protein expression.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 6416
    Illegal XbaI site found at 7672
    Illegal PstI site found at 3597
    Illegal PstI site found at 6353
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6416
    Illegal NheI site found at 9871
    Illegal PstI site found at 3597
    Illegal PstI site found at 6353
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6416
    Illegal BglII site found at 2228
    Illegal BglII site found at 8164
    Illegal XhoI site found at 4292
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 6416
    Illegal XbaI site found at 7672
    Illegal PstI site found at 3597
    Illegal PstI site found at 6353
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 6416
    Illegal XbaI site found at 7672
    Illegal PstI site found at 3597
    Illegal PstI site found at 6353
    Illegal AgeI site found at 1504
    Illegal AgeI site found at 7172
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 9834


[edit]
Categories
Parameters
None