Part:BBa_K4131008
pfa B gene (Shewanella pneumatophori), codon optimized for E.Coli expression
In Moritella marina and Shewanella pneumatophori, the pfa gene cluster encodes five open reading frames, namely pfa A, pfa B, pfa C, pfa D, and pfa E. Each gene contains up to several functional domains to perform carboxylation, dehydration, and reduction of the ketoacyl group and hydroxyl groups in the PKS pathway. In Moritella marina, the pfa genes work together to generate DHA, while Shewanella pneumatophori generates EPA. The exact functions of pfa A-D genes are unclear. To the best of our knowledge, the pfa B gene is the key molecule to determine the final product (e.g. EPA and DHA).
In our project, the pfa B (Shewanella pneumatophori) is coepxressed with pfa A, pfa C, pfa D, and pfa E to form pfa megaenzyme and generate EPA.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1305
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 297
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 1515
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