Part:BBa_K4131006

Original pfa D gene (Moritella marina)

In Moritella marina and Shewanella pneumatophori, the pfa gene cluster encodes five open reading frames, namely pfa A, pfa B, pfa C, pfa D, and pfa E. Each gene contains up to several functional domains to perform carboxylation, dehydration, and reduction of the ketoacyl group and hydroxyl groups in the PKS pathway. In Moritella marina, the pfa genes work together to generate DHA, while Shewanella pneumatophori generates EPA. The exact functions of pfa A-D genes are unclear. To the best of our knowledge, the pfa B gene is the key molecule to determine the final product (e.g. EPA and DHA).

In our project, the pfa D is coepxressed with pfa A, pfa B, pfa C, and pfa E to form pfa megaenzyme and generate DHA.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 377
  Illegal PstI site found at 1079
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1302
  Illegal PstI site found at 377
  Illegal PstI site found at 1079
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 240
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 377
  Illegal PstI site found at 1079
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 377
  Illegal PstI site found at 1079
  
• 1000
  COMPATIBLE WITH RFC[1000]