Part:BBa_K4131005

Original pfa C gene (Moritella marina)

In Moritella marina and Shewanella pneumatophori, the pfa gene cluster encodes five open reading frames, namely pfa A, pfa B, pfa C, pfa D, and pfa E. Each gene contains up to several functional domains to perform carboxylation, dehydration, and reduction of the ketoacyl group and hydroxyl groups in the PKS pathway. In Moritella marina, the pfa genes work together to generate DHA, while Shewanella pneumatophori generates EPA. The exact functions of pfa A-D genes are unclear. To the best of our knowledge, the pfa B gene is the key molecule to determine the final product (e.g. EPA and DHA).

In our project, the pfa C is coepxressed with pfa A, pfa B, pfa D, and pfa E to form pfa megaenzyme and generate DHA.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 3490
  Illegal XbaI site found at 4746
  Illegal PstI site found at 671
  Illegal PstI site found at 3427
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 3490
  Illegal PstI site found at 671
  Illegal PstI site found at 3427
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 3490
  Illegal BglII site found at 5238
  Illegal XhoI site found at 1366
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 3490
  Illegal XbaI site found at 4746
  Illegal PstI site found at 671
  Illegal PstI site found at 3427
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 3490
  Illegal XbaI site found at 4746
  Illegal PstI site found at 671
  Illegal PstI site found at 3427
  Illegal AgeI site found at 4246
  
• 1000
  COMPATIBLE WITH RFC[1000]