Coding

Part:BBa_K4131001

Designed by: Chun Ying Yu   Group: iGEM22_CCU_Taiwan   (2022-10-09)


AccBC gene (C. glutamicum), codon optimized for E. Coli expression

The acetyl-CoA carboxylase of C. glutamicum is composed of a biotin carboxylase (AccBC), a biotin carboxyl carrier protein (AccE), and a transcarboxylase (AccD1). They carboxylated acetyl-CoA into malonyl-CoA, which is necessary for the elongation of nascent fatty acid. The carboxylation occurs in two steps. In the first step, the ATP-dependent biotin carboxylase (AccBC) catalyzes the transfer of a carboxyl group to biotin, which is linked to the biotin carboxyl carrier protein (AccE). In the second step, the transcarboxylase (AccD1) transferred the carboxyl group from carboxybiotin to acetyl-CoA to form malonyl-CoA.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1331
    Illegal XhoI site found at 80
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 688
    Illegal NgoMIV site found at 1062
    Illegal NgoMIV site found at 1552
    Illegal AgeI site found at 426
    Illegal AgeI site found at 928
    Illegal AgeI site found at 1711
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None