Composite
Part:BBa_K4129123
Designed by: Magnus Haahr Group: iGEM22_DTU-Denmark (2022-10-09)
The fungal synthetic expression system with FunsTF05 and 6xLexO-Pmin
Fungal synthetic expression system using FunsTF05
In this fungal synthetic expression system (SES) FunsTF05 was constitutively expressed from PgpdA. The FunsTF05, which contains a LexA DNA-binding domain, would be able to bind to the LexO binding sites for the minimal promoter fused with LexO binding sites (6xLexO-Pmin). The efficiency of the FunsTF05 activation was measured by the reporter gene mCherry, which is expressed from the 6xLexO-Pmin.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 4591
Illegal XbaI site found at 6504
Illegal PstI site found at 141 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 911
Illegal PstI site found at 141 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 145
Illegal BglII site found at 3258
Illegal BamHI site found at 2920
Illegal BamHI site found at 5785
Illegal XhoI site found at 701
Illegal XhoI site found at 1083
Illegal XhoI site found at 3113
Illegal XhoI site found at 3550
Illegal XhoI site found at 4455 - 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 4591
Illegal XbaI site found at 6504
Illegal PstI site found at 141 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 4591
Illegal XbaI site found at 6504
Illegal PstI site found at 141
Illegal NgoMIV site found at 6421
Illegal AgeI site found at 1290
Illegal AgeI site found at 1438 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1117
Illegal BsaI.rc site found at 6135
Illegal SapI site found at 323
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Categories
Parameters
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