Composite

Part:BBa_K4129123

Designed by: Magnus Haahr   Group: iGEM22_DTU-Denmark   (2022-10-09)


The fungal synthetic expression system with FunsTF05 and 6xLexO-Pmin

Fungal synthetic expression system using FunsTF05

In this fungal synthetic expression system (SES) FunsTF05 was constitutively expressed from PgpdA. The FunsTF05, which contains a LexA DNA-binding domain, would be able to bind to the LexO binding sites for the minimal promoter fused with LexO binding sites (6xLexO-Pmin). The efficiency of the FunsTF05 activation was measured by the reporter gene mCherry, which is expressed from the 6xLexO-Pmin.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 4591
    Illegal XbaI site found at 6504
    Illegal PstI site found at 141
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 911
    Illegal PstI site found at 141
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 145
    Illegal BglII site found at 3258
    Illegal BamHI site found at 2920
    Illegal BamHI site found at 5785
    Illegal XhoI site found at 701
    Illegal XhoI site found at 1083
    Illegal XhoI site found at 3113
    Illegal XhoI site found at 3550
    Illegal XhoI site found at 4455
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 4591
    Illegal XbaI site found at 6504
    Illegal PstI site found at 141
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 4591
    Illegal XbaI site found at 6504
    Illegal PstI site found at 141
    Illegal NgoMIV site found at 6421
    Illegal AgeI site found at 1290
    Illegal AgeI site found at 1438
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1117
    Illegal BsaI.rc site found at 6135
    Illegal SapI site found at 323


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Categories
Parameters
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