Plasmid

Part:BBa_K4129035

Designed by: Giovanni Carlo Gendarini   Group: iGEM22_DTU-Denmark   (2022-10-11)


Transient expression system for BBa_K4129006

Plasmid to transiently express BBa_K4129006 in Aspergillus niger.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 5348
    Illegal EcoRI site found at 7625
    Illegal EcoRI site found at 9450
    Illegal XbaI site found at 3527
    Illegal XbaI site found at 4293
    Illegal XbaI site found at 4775
    Illegal XbaI site found at 5543
    Illegal XbaI site found at 7430
    Illegal XbaI site found at 8198
    Illegal XbaI site found at 8680
    Illegal SpeI site found at 34
    Illegal SpeI site found at 160
    Illegal SpeI site found at 3710
    Illegal SpeI site found at 3721
    Illegal SpeI site found at 4085
    Illegal SpeI site found at 4217
    Illegal SpeI site found at 4341
    Illegal PstI site found at 6492
    Illegal PstI site found at 10288
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 5348
    Illegal EcoRI site found at 7625
    Illegal EcoRI site found at 9450
    Illegal NheI site found at 10944
    Illegal SpeI site found at 34
    Illegal SpeI site found at 160
    Illegal SpeI site found at 3710
    Illegal SpeI site found at 3721
    Illegal SpeI site found at 4085
    Illegal SpeI site found at 4217
    Illegal SpeI site found at 4341
    Illegal PstI site found at 6492
    Illegal PstI site found at 10288
    Illegal NotI site found at 3566
    Illegal NotI site found at 10935
    Illegal NotI site found at 10989
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 5348
    Illegal EcoRI site found at 7625
    Illegal EcoRI site found at 9450
    Illegal BglII site found at 1915
    Illegal BglII site found at 9352
    Illegal BamHI site found at 2175
    Illegal BamHI site found at 9490
    Illegal BamHI site found at 10026
    Illegal XhoI site found at 5889
    Illegal XhoI site found at 6606
    Illegal XhoI site found at 7084
    Illegal XhoI site found at 9886
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 5348
    Illegal EcoRI site found at 7625
    Illegal EcoRI site found at 9450
    Illegal XbaI site found at 3527
    Illegal XbaI site found at 4293
    Illegal XbaI site found at 4775
    Illegal XbaI site found at 5543
    Illegal XbaI site found at 7430
    Illegal XbaI site found at 8198
    Illegal XbaI site found at 8680
    Illegal SpeI site found at 34
    Illegal SpeI site found at 160
    Illegal SpeI site found at 3710
    Illegal SpeI site found at 3721
    Illegal SpeI site found at 4085
    Illegal SpeI site found at 4217
    Illegal SpeI site found at 4341
    Illegal PstI site found at 6492
    Illegal PstI site found at 10288
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 5348
    Illegal EcoRI site found at 7625
    Illegal EcoRI site found at 9450
    Illegal XbaI site found at 3527
    Illegal XbaI site found at 4293
    Illegal XbaI site found at 4775
    Illegal XbaI site found at 5543
    Illegal XbaI site found at 7430
    Illegal XbaI site found at 8198
    Illegal XbaI site found at 8680
    Illegal SpeI site found at 34
    Illegal SpeI site found at 160
    Illegal SpeI site found at 3710
    Illegal SpeI site found at 3721
    Illegal SpeI site found at 4085
    Illegal SpeI site found at 4217
    Illegal SpeI site found at 4341
    Illegal PstI site found at 6492
    Illegal PstI site found at 10288
    Illegal NgoMIV site found at 6626
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 564
    Illegal BsaI site found at 929
    Illegal BsaI site found at 9219
    Illegal BsaI site found at 10079
    Illegal BsaI.rc site found at 2268
    Illegal BsaI.rc site found at 3754


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Categories
Parameters
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